BMC Infectious Diseases is an open access, peer-reviewed journal that considers articles on all aspects of the prevention, diagnosis and management of infectious and sexually transmitted diseases in humans, as well as related molecular genetics, pathophysiology, and epidemiology. BMC Infectious Diseases is an open access, peer-reviewed journal that considers articles on all aspects of the prevention, diagnosis and management of infectious and sexually transmitted diseases in humans, as well as related molecular genetics, pathophysiology, and epidemiology. Valizadeh A, et al. Abbreviation 2: BMC Infect Dis ISSN: 1471-2334 (Online) Other Information: Country: England Publisher: London : BioMed Central, [2001- Website: http://www.biomedcentral.com/bmcinfectdis/ http://www.pubmedcentral.nih.gov/tocrender.fcgi?journal=36 https://doi.org/10.1186/s12879-022-07835-z, DOI: https://doi.org/10.1186/s12879-022-07835-z. By using this website, you agree to our Microchem J. The impact factor (IF) or journal impact factor (JIF) of an academic journal is a scientometric factor based on the yearly average number of citations on articles published by a particular journal in the last two years. Based on the biopsychosocial model, psychosomatic medicine, which has paid great attention to the integrity of the body and disease, believes that the development of chronic non-infectious diseases, including type 2 diabetes mellitus (DM), is closely related to psychosocial factors [].However, it is difficult to systematically describe the progression of these diseases, and current diagnostic . The specificity of RT-qPCR was achieved through a Melt curve which included Leishmania species serial dilution. Leishmania and the Model of Predominant Clonal Evolution. 1). Globally, respiratory viral disease causes substantial morbidity and mortality with significant economic impact [].Prior to SARS-CoV-2, influenza virus and respiratory syncytial virus (RSV) were the respiratory pathogens most commonly responsible for severe disease causing hospitalization [].Diagnosis of these pathogens remains important to guide clinical management, to detect and manage . Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. To overcome these problems, over the past decades Nanoparticle (NP)-based diagnostic approaches have provided highly attractive [4, 10], easy to perform, precise and efficient approaches for sensing and detecting small amounts of biological material, such as DNA, without the need for amplifying the genome [11]. The ITS2 sequence was selected to serve as a template. BMC Infect Dis. The journal charges up to: 1690 GBP; as publication fees (article processing charges or APCs). . The ISSN of International Journal of Infectious Diseases journal is 12019712, 18783511 . Positive controls were provided via cultivating the standard strains of L. major (MHOM/IR/75/ER), L. tropica (MHOM/SU/74/K27) and L. infantum (MCAN/IR/97/LON490), which were obtained from Pasteur Institute of Iran. Infectious diseases of poverty Impact factor 3.063 (2022) International Journal of Biological Macromolecules Impact factor 6.953 (2022) International Journal of Nanomedicine Impact factor . Zitat Wang Y et al (2021) Inhibition of Streptococcus mutans biofilms with bacterial-derived outer membrane vesicles. UpToDate. Aithal S, et al. BMC is part of Springer Nature. Therefor the high specificity and sensitivity along with the extremely low threshold of detection are the cumulative result of ITS2 and AuNPs properties. 2014;96:5661. Parasites are transmitted by different sandflies genera worldwide as Phlebotomine, Lutzomyia and Nissomya genera [3, 4]. The increasing rate of MDR bacterial infections, mainly ESBL producers of LRTIs causes difficulty in disease management, leading to high morbidity and mortality of PLHA. 2017;57:129. Sattarahmady N, Kayani Z, Heli H. Highly simple and visual colorimetric detection of Brucella melitensis genomic DNA in clinical samples based on gold nanoparticles. Mobed A, et al. Case: 23-year-old female patient applied to the emergency service of an external center with complaints of sudden onset of headache . These nanobiosensors do not require elaborate approaches for performance and detection or simple agglomeration is convenient via naked eye. For the PCR-based agglutination approach, the minimum of threshold was 16fg/L (Fig. Leishmaniasis is a prevalent vector-borne infection which is caused by 21 zoonotic Leishmania spp in more than 90 countries [1, 2]. The characteristics of the HZ disease are summarized in Table 2. BMC Infectious Diseases A 20-mer single-stranded oligonucleotide was selected and thiolated at the 5 end to serve as probe which was ordered from the Bioneer Company (South Korea). The 2021 impact factor of BMC Infectious Diseases is 3.9, making it among the top 4% journals. Nanomaterial-assisted signal enhancement of hybridization for DNA biosensors: a review. . Connect with NLM. Each L. major, L. tropica and L. infantum standard strain was cultured in the Roswell Park Memorial Institute (RPMI) medium supplemented with 15% fetal bovine serum (FBS), 100IU of penicillin and 100g/mL of streptomycin at 25C in a shaker incubator. Conventional diagnostic approaches such as culture, microscopic examination and serology methods are labor-working and have poor sensitivity for the parasite identification. The first time 4.4L and for the next subsequent times 8.8L of high-salt solution was added. The main research topics published in this journal include Internal medicine, Immunology, Population . The single and probe-conjugated AuNPs were subjected to UVvis spectroscopy, DLS and TEM analysis. Sensors. Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. Gold NPs (AuNPs) have provided novel alternative approach in the field of diagnostics owing to their unique optical, electric and physicochemical features along with containing a variety of functional chemical groups [12]. 5). The previous impact factors of the journal are displayed in the corresponding graph. Sattarahmady N, et al. Other variant title: BioMed Central infectious diseases Other variant title: Infectious diseases Country: United Kingdom Record information Last modification date: 06/02/2021 Type of record: Confirmed ISSN Center responsible of the record: ISSN National Centre for the UK My Tools Share BMC Infectious Diseases 2022 22 :849 Research Published on: 14 November 2022 Full Text PDF Molecular characterization and genotyping of hepatitis C virus from Sudanese end-stage renal disease patients on haemodialysis Hepatitis C virus (HCV) is a global public health problem, with ~ 11 million people in Africa infected. The International Journal of Infectious Diseases (IJID) is published monthly by the International Society for Infectious Diseases. BMC Infect Dis JUNE 7, 2022 Multidrug-resistant bacteria with ESBL genes: a growing threat among people living with HIV/AIDS in Nepal Maharjan R, Bastola A, . We report herein the case of a pediatric patient diagnosed and . All its journals are published online only. Privacy Join the conversation about this journal Quartiles Andreadou M, et al. Other variant title: BioMed Central infectious diseases Other variant title: Infectious diseases Country: United Kingdom Medium: Online Record information Last modification date: 06/02/2021 Type of record: Confirmed ISSN Center responsible of the record: ISSN National Centre for the UK We believe, however, that additional comments could be made. The article by Reich et al. Journal ISSN: 1471-2334 About Bmc Infectious Diseases BMC Infectious Diseases is an open access journal publishing original peer-reviewed research articles in all aspects of the prevention, diagnosis and management of infectious and sexually transmitted diseases in humans, as well as related molecular genetics, pathophysiology, and epidemiology. Akhoundi M, et al. The oligonucleotide was thoroughly analyzed via bioinformatics tools ensuring that would solely bind to Leishmania spp DNA. The probe is designed to specifically detect L. tropica, L. major and L. infantum. 2022;128: 106062. Cookies policy. ISSN: 1471--2334. It was demonstrated that conjugated NPs had a higher resistance toward MgCl2 induced aggregation when compared with NPs singly (Fig. M. S. reports support for attending meetings and/or travel from Gilead Sciences; and participation on an advisory board (payment to institution) for Gilead Sciences, MSD, and ViiV Health Care. Previous Journal: Euphytica; Netherlands journal of plant breeding Abbreviation, Next Journal: Journal of cardiopulmonary rehabilitation and prevention Abbreviation, Euphytica; Netherlands journal of plant breeding Abbreviation, Journal of cardiopulmonary rehabilitation and prevention Abbreviation, Annales de dermatologie et de venereologie, Journal of microbiology, immunology, and infection, The Canadian journal of infectious diseases, European journal of clinical microbiology & infectious diseases. Owing to the accuracy, high sensitivity and specificity and validity of the adopted AuNPs-probe conjugated biosensor designed in this study, its application for the screening of suspicious patients is promising, particularly in lower-developed areas. Diagn Microbiol Infect Dis. Cite this article. 2020;13(1):112. However, in the second approach we strived to PCR amplify the target DNA, while it was captured by the AuNP-probes conjugate. Talanta. 10.485 - 2-year Impact Factor (2021) 6.771 - 5-year Impact Factor (2021) 1.762 - Source Normalized Impact per Paper (SNIP) 1.849 - SCImago Journal Rank (SJR) Speed 8 days to first decision for all manuscripts (Median) 31 days to first decision for reviewed manuscripts only (Median) Usage 1,531,708 Downloads (2021) 1,578 Altmetric mentions (2021) 2021;188(3):19. ( Lippincott Williams & Wilkins ) Purpose of review: Increased emergence of bacterial resistance and the decline in newly developed antibiotics have necessitated the reintroduction of previously abandoned antimicrobial agents active against multidrug . ITSs are removed from the primary transcript by snoRNAs and multiple enzymes [23, 24]. Infectious Diseases of Poverty is an open access, peer-reviewed journal publishing topic areas and methods that address essential public health questions relating to infectious diseases of poverty. Despite this, surveillance relies heavily on these laboratory results. This study described diarrhoeal diagnostic practices and aetiological agents of diarrhoea in patients admitted to three South African . AuNPs-based DNA biosensors coated with single-stranded DNA oligonucleotides via thiol bonds have gained widespread applications and become more significant in the fields of diagnostics, forensics and patient follow-up [19, 20]. So objetivos deste artigo i) demonstrar os primeiros resultados da pesquisa de sistematizao de bibliografias que constituem interlocues entre a Geografia e as reas da Sade, tendo . It has an SJR impact factor of 1,278. The index is based on the set of the scientist's most cited papers and the number of citations that they have received in other publications. BMC Infectious Diseases This journal has been awarded the DOAJ Seal. LTD, Korea). Gold nanomaterials for oral cancer diagnosis and therapy: advances, challenges, and prospects. Using the integrated platform of the vaccination campaign of Lombardy Region (Italy), after the exclusion of 24,188 individuals not beneficiaries of the Regional Health Service . Dis. Sexually transmitted diseases While immunoassay methods have been employed to diagnose leishmaniasis such as ELISA, the tests have exhibited cross-reactivity with other parasitic agents namely Trypanosoma and Plasmodium spp., rendering the immunoassay methods rather non-specific. 2012;6(2): e1511. J Iran Chem Soc. BMC Infectious Diseases is an open access, peer-reviewed journal that considers articles on all aspects of the prevention, diagnosis and management of infectious and sexually transmitted diseases in humans, as well as related molecular genetics, pathophysiology, and epidemiology. All VL were L. infantum and All CL were L. major. The red color indicates dispersed nanoparticles whereas the purple color indicates aggregated nanoparticles. The solution was centrifuged at 16,000g for 30s at 4C. BioNanoScience. 0:07 1:22 BMC 20th anniversary This year BMC is celebrating its 20 th year anniversary. A journal impact factor is frequently used as a proxy for the relative importance of a journal within its field. Google Scholar. Charyyeva A, et al. There are, however, some facilities such as a dynamic light scattering machine which may seem less routine; however, in comparison to qPCR etc. A recent study using AuNP-probe and molecular methods outlined detection limits of 102 parasites/mL (0.0147ng/L) for the diagnosis of VL among human immunodeficiency virus (HIV) infected patients [29]. Publishing with this journal. Background Stool samples submitted for diagnostic testing represent a proportion of diarrhoeal cases seeking healthcare, and an even smaller proportion of diarrhoeal cases in the community. From manufacturing vantage point, nanoparticle preparation and validating the final products require basic lab equipment, for instance a spectrophotometer, which can be found in every laboratory worldwide. Samples containing amastigote form of Leishmania spp were considered as positive controls. Due to positive serum and cerebrospinal fluid myelin oligodendrocyte glycoprotein (MOG) antibodies, we made the diagnosis of . Determining the threshold of detection using the PCR-based agglutination approach. An International Standard Serial Number (ISSN) is a unique code of 8 digits. J Drug Deliv Sci Technol. There are also other factors to sider for example, H-Index, Self-Citation Ratio, SJR (SCImago Journal Rank Indicator) and SNIP (Source Normalized Impact per Paper). Promising antibacterial effect of impregnated nanofiber mats with a green nanogel against clinical and standard strains of Pseudomonas aeruginosa and Staphylococcus aureus. Biotechnol Appl Biochem. After decanting the supernatant, the mixture was washed with 500L wash buffer, (NaCl 1.5Mm, 0.1% SDS in PBS Buffer) and centrifuged at 16,000g at 4C for 30s. The supernatant was decanted and the pellet containing AuNP-probe conjugate was re-suspended with 200L wash buffer and stored at 4C while wrapped around aluminum foil until further use. 2016;161:4853. The lowest DNA concentration at which the target DNA was detected for all Leishmania spp included 32fg/L, which was equivalent to 0.3 parasite/L (Fig. To this end, here we aimed to design and construct an AuNP-conjugated probe as a Geno-sensor to capture the ITS2 (Internal transcribed spacer) of Leishmania species to visually detect leishmaniasis utilizing two approaches. Performance of a universal PCR assay to identify different Leishmania species causative of Old World cutaneous leishmaniasis. California Privacy Statement, J Microbiol Methods. 3509~3520 ISSN: 2302-9285, DOI: 10.11591/eei.v11i6.4225 3509 Machine learning approaches in the diagnosis of infectious diseases: a . Pharmaceutics. Tibayrenc M, Ayala FJ. The first method was carried out by hybridization of 10L of DNA with 4 L of probe and addition of 5 L of 0.2N HCl (non-amplification method). Follow NCBI. 2021;218: 105888. PubMedGoogle Scholar. Biochem Eng J. Bern C. Visceral leishmaniasis: clinical manifestations and diagnosis. Restriction fragment length polymorphism, PCR and RT-qPCR are further molecular approaches; however, they are cost-intensive as extravagant high-tech facilities are required [5, 18, 19]. 2021;11(3):67886. Dis. Emerging Infectious Disease journal ISSN: 1080-6059. Correspondence to Although most molecular diagnostic approaches have great sensitivity and specificity, fast performance and low contamination risk, these methods require skilled personnel, specialized reagents and expensive high-tech facilities which is a significant downside to their application particularly in low-income countries [9]. Microorganisms. Despite data suggesting that the pediatric population is less affected by coronavirus disease-19 infection, new concerns have been raised owing to reported cases with hyperinflammatory conditions such as Kawasaki disease. Google Scholar h-Index 2022 h5-index All samples were collected from southern Iran.The samples were previously analyzed via Giemsa staining of smears of lesions (observed by light microscope), biopsies or blood smears and confirmed whit molecular methods. Print ISSN: 0937-4477 . If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. All authors read and approved the final manuscript. ADD TO MY LIST. The International Agency for Research on Cancer estimates that about 20% of cancers in developed countries and close to 30% of cancers in developing countries have an infectious etiology. Moreover, the size of NPs using TEM analysis included~20nm (Fig. In terms of detection, NPs offer several straight forward approaches such as change of color upon aggregation via induction with acidic solutions [13, 14]. have conceptualized, supervised and performed the work, AI.G., S.A., M.K., S.S. and A.Y. Any journal impact factor or scientometric indicator alone will not give you the full picture of a science journal. In this method, 5 L of PCR products was supplemented with 20 L of NPs and 2.5 L of HCl (0.2M). Sensitivity for detection of visceral leishmaniasis (VL) for non-amplification and amplification methods included 96% and 100%, respectively and for cutaneous leishmaniasis (CL) included 98% and 100%, respectively. URL: www.biomedcentr http://www.biomedcentral.com/bmcinfectdis/, URL: www.pubmedcentr http://www.pubmedcentral.nih.gov/tocrender.fcgi?journal=36, SUDOC: www.sudoc.fr/06 http://www.sudoc.fr/06030376X, Wikidata: www.wikidata.or http://www.wikidata.org/entity/Q15759919, FATCAT: fatcat.wiki/con https://fatcat.wiki/container/gryfylmjtnau5b3kr62ex5g6yy, Google: www.google.com/ https://www.google.com/search?q=ISSN+%221471-2334%22, Bing: www.bing.com/se https://www.bing.com/search?q=ISSN+%221471-2334%22, Yahoo: search.yahoo.co https://search.yahoo.com/search?p=ISSN%20%221471-2334%22, CROSSREF: search.crossref https://search.crossref.org/?q=+1471-2334, MIR@BEL: reseau-mirabel. Nanobiosensors could be also used as a point-of-care testing as they are extremely easy to transport and the detection does not require any laboratory equipment. PubMed Liu J, et al. The Toxoplasma gondii, Escherichia coli, cryptosporidium parvum and Candida albicans served as a negative control species. Rovery C, Brouqui P, Raoult D. . Zhang Q, et al. Part of Introduction We aimed to assess harms (post-vaccine myocarditis and pericarditis) and benefits (preventing severe disease) of COVID-19 vaccination. Ensuing hybridization of probe with the target DNA, the solution was supplemented with HCl, rendering NPs to aggregate if not hybridized with the target DNA. U: UpToDate, Post TW ur. Also Check H-Index, SCImago journal rank and journal impact factor 2021. The incubation period of an infectious disease is defined as the elapsed time between the exposure to the pathogen and the onset of symptoms. Mann S, et al. Over the last decade, molecular-based approaches such as polymerase chain reaction (PCR) and real-time PCR have been employed for the rapid, accurate and sensitive diagnostic purposes as they have enabled researchers to focus on specific genetic loci which were unique to the pathogen under investigation [8]. The optical properties of gold dispersions were investigated using UVVis spectrophotometer (UVIKON 923 UVVis spectrophotometer). [8], the detection limits of Leishmania spp were 1.2ng, 0.07ng, 11.5ng and 0.07ng/ L, respectively. Microchim Acta. Publisher: BioMed Central. PubMed Researchers may also consider the practical aspect of a journal such as publication fees, acceptance rate, review speed. The ISSN of BMC Infectious Diseases journal is 14712334 . CAS Mol Aspects Med. BMC Infect Dis; BMC Infectious Diseases Vols. Ruang-Areerate T, et al. This is particularly important for faraway regions, where medical staff do not have access to laboratory equipment. Citrate-capped AuNPs with an average diameter of about 20nm were synthesized using previous method described by [15, 16]. Second method was followed by polymerase chain reaction (PCR) amplification using thiolated primer, 5 L of AuNP and 5 L of 0.2N HCl. The abbreviation of the journal title " BMC infectious diseases " is " BMC Infect. Title proper: BMC infectious diseases. Provided by the Springer Nature SharedIt content-sharing initiative. Si se introduce en la casilla de bsqueda cualquier ISSN el sistema localizar en qu bases de datos de las contempladas en la matriz est indizada la revista, est recogida o no en MIAR. PCR diagnosis and characterization of Leishmania in local and imported clinical samples. Waltham, MA: UpToDate, 2021. Parasit Vectors. In this study, we developed a simple assay based on a non-cross-linking hybridization method, where the aggregation of AuNPs-probe conjugate was induced by an increasing acid concentration. An electrophoresis approach with online thermal lens detection to monitoring DNA surface coatings on gold nanoparticles. The developed Leishmania spp AuNP-probe conjugate assay had a detection limit of 32fg/L of unamplified L. major, L. tropica and L. infantum DNA fragments. The informed consent was obtained from all subjects and/or their legal guardian(s). 2C). The mean time elapsed (SD) from the onset of the rash at enrollment was 21.3 (17.6) days. The oligonucleotide attached to the nanoparticles serve as the probe that captures the target DNA of Leishmania spp. 2022;15:100333. Article In contrast, the negative samples and other bacterial DNA, such as T. gondii, E. coli and C. albicans contained non-complementary targets which did not prevent AuNPs-probe aggregation, resulting in a color change from red to purple with a corresponding red shift in surface plasmon resonance from 528580nm. The results of this investigation revealed that sensitivity of amplification method was the same as RT-qPCR, while that of non-amplification method was lower. 2022;173: 106961. As for the negative controls, samples lacking the target DNA were subjected to the same process. Thus in this study, ITS2 conserved sequence was adopted and the sensitivity and specificity of conjugation method was similar to those of PCR and qPCR amplification methods giving rapid results. 2021;13(4):491. BMC chemistry Impact factor 2.094 (2022) Chemical Reviews Impact factor 60.622 (2022) Chemical Society Reviews Impact factor . Red color indicates AuNPs-probes dispersion and purplish color indicates aggregated nanoparticle. Biological psychiatry. 4). In the first approach, we strived to capture the target DNA with AuNP probe. Front Cell Infect Microbiol. The 2021 impact factor of Bmc Infectious Diseases is 3.8, making it among the top 3% journals. Gilad Lazarovits, Orit Gefen, Noga Cahanian, Karen Adler, Ronen Fluss, Irit Levin-Reisman, Irine Ronin, Yair Motro, Jacob Moran-Gilad, Nathalie Q Balaban, Jacob Strahilevitz, Prevalence of Antibiotic Tolerance and Risk for Reinfection Among Escherichia coli Bloodstream Isolates: A Prospective Cohort Study, Clinical Infectious Diseases, Volume 75, Issue 10, 15 November 2022, Pages 1706-1713 . Nanobiosensor platforms have emerged as convenient and promising approaches with remarkable efficacy for the diagnosis of infectious diseases. The results of the two approaches were comparatively analyzed. Although the selection of oligo probe was highly important in the tests efficacy, the nanobiosensors also exploit the advantages of AuNPs. Article Once boiled, 5mL of 25.9mM trisodium citrate were added under vigorous stirring which was continued for 30min for color change to deep red indicating the formation of AuNPs. J Mol Recognit. Clinical outcomes of spontaneous bacterial peritonitis due to extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella species: A retrospective matched case-control study. The mean diameter values of particle size (PS) and particle size distribution (SPAN) of the AuNPs were determined using dynamic light scattering (DLS) (scatteroscope, K-ONE NANO. In other words, the impact factor of 2021 is the average of the number of cited publications divided by the citable publications of a journal. EID's impact factor indicates that articles published in the journal during the last 2 years have been cited an average of more than 16 times during the past year. BMC Infectious Diseases Volumes and issues Volume 22 January - December 2022 December 2022, issue 1 January 2022, issue 1 Innovating with HIV self-testing for impact in southern Africa: Lessons learned from the STAR (Self-Testing AfRica) Initiative This is a supplement Volume 21 January - December 2021 December 2021, issue 1 September 2021, issue 2 Here, we sought to select an oligonucleotide with high conservancy among different species of Leishmania to serve as a specific probe with scavenging ability through heterogeneous samples to bind to its target DNA. Analysis of available bioinformatics databases has revealed different subunits of the rRNAs of L. infantum located in different chromosomes and are not arranged in a head-to-tail tandem array. A novel non-amplification assay for the detection of Leishmania spp. The 2021 impact factor of Bmc Infectious Diseases is 3.8, making it among the top 3% journals. Logistic . The NPs deciphered the highest absorption at 524nm wavelength (Fig. In this method, colorimetric assay was employed. However, the remaining samples namely blank, negative control and those with DNA concentrations lower than the threshold, reflected the highest absorbance at 524nm to 610nm (Fig. Published in BMC Infectious Diseases ISSN 1471-2334 (Online) Publisher BMC Country of publisher United Kingdom LCC subjects Medicine: Internal medicine: Infectious and parasitic diseases Website https://bmcinfectdis.biomedcentral.com. To do so, 1L of MgCl2 (5mM) was added to 5L of AuNP-probe conjugate and non-conjugated nanoparticle separately. in clinical samples using gold nanoparticles. Nicolas L, Milon G, Prina E. Rapid differentiation of Old World Leishmania species by LightCycler polymerase chain reaction and melting curve analysis. Clinical samples (n=101) encompassing 25 VL and 76 CL species along with 18 non-Leishmania skin wounds were investigated. BMC Infectious Diseases focuses its scope in these topics and keywords: hiv, tuberculosis, patients, viral, south, incidence, comparison, antiretroviral, retrospective, infections, . genomic DNA: Visual and spectrophotometric detections. NCBI database was used for primer BLAST and MEGA6 confirmed the primers specifically for the detection of Leishmania species. DOI . Student Research Committee, Fasa University of Medical Sciences, Fasa, Iran, Someye Deris,Sahar Samsami&Ava Yazdanpanah, Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Fasa University of Medical Sciences, Fasa, Iran, Noncommunicable Diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran, Abdolmajid Ghasemian,Maryam Kohansal&Ali Ghanbariasad, Biotechnology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran, Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Fasa University of Medical Sciences, Fasa, Iran, Department of Medical Nanotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Unit 23, 2nd Floor, Almass Building, Alley 29, Ghasrodasht St., Shiraz, Iran, You can also search for this author in The efficacy of AuNP-probe conjugate nanobiosensor in non-amplification and amplification forms for the diagnosis of leishmaniasis. J. S. F. reports support for this work from Merck. Additionally, parasites isolation requires their cultivation is a time-consuming approach with low sensitivity [7]. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. . Shrestha UT. SCImago Journal Rank (SJR indicator) is a measure of scientific influence of scholarly journals that accounts for both the number of citations received by a journal and the importance or prestige of the journals where such citations come from. Next, 100L phosphate buffer (100mM, pH:7) and 11L sodium dodecyl sulfate (SDS) (10%) were added to the mixture of probe and NPs and placed in shaker for 30min. Talanta. Randomly positioned gold nanoparticles as fluorescence enhancers in apta-immunosensor for malaria test. Rezvan H, HamoonNavard S. An overview on Leishmania diagnosis. The AuNPs solution was stored at 4C in a dark environment for further use. However, this method was promising because of no need for any equipment, high specificity, enough sensitivity, low cost and rapidity (less than 30min) to complete after genomic DNA extraction. These include various aspects of the biology of pathogens and vectors, diagnosis and detection, treatment and case management, epidemiology and modeling, zoonotic hosts and animal reservoirs . . Note: impact factor data for reference only. Thus, we opted for designing an AuNP-based biosensor to improve drawbacks, as they contain advantages such as easy production and cost-effectiveness. Furthermore, the quantitative real-time PCR (RT-qPCR) technique was exploited as the reference method of detection. Trends Parasitol. 2006; 6: 60. Potential Conflicts of interest. Schematic illustration of the process; A dispersal of hybridized AuNPs-probes in the solution and B AuNPs-probes aggregation upon the addition of HCl in samples devoid of target DNA. 10.485 - 2-year Impact Factor (2021) 6.771 - 5-year Impact Factor (2021) 1.762 - Source Normalized Impact per Paper (SNIP) 1.849 - SCImago Journal Rank (SJR) Speed 8 days to first decision for all manuscripts (Median) 31 days to first decision for reviewed manuscripts only (Median) Usage 1,531,708 Downloads (2021) 1,578 Altmetric mentions (2021) Deciphered the highest absorption at 524nm wavelength ( Fig serology methods are labor-working and have poor for! At enrollment was 21.3 ( 17.6 ) days approaches in the corresponding graph and cost-effectiveness previous impact factors the... At 16,000g for 30s at 4C in a dark environment for further use was highly in... The first time 4.4L and for the parasite identification Join the conversation about this journal has awarded! 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